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MB Sample ID: SA185560

Local Sample ID:	Sg_tagatose_4
Subject ID:	SU002060
Subject Type:	Bacteria
Subject Species:	Streptococcus gordonii DL1 Challis / Streptococcus mutans UA159 / Streptococcus oralis ATCC9811

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MS

Sample Preparation:

Sampleprep ID:	SP002066
Sampleprep Summary:	Ribitol was added as an internal standard for normalization, then acetonitrile was added to remove protein. After thoroughly mixing the samples, centrifugation was performed. The upper 1.6 ml was evaporated in a centrifugal concentrator and freeze-dried overnight. Methoxyamine hydrochloride in pyridine and N-methyl-N-trimethylsilyltrifluoroacetamide were added to the samples as derivatizing agents, then they were thoroughly mixed and centrifuged, after which the upper 50 μl of each sample was transferred to a vial.

Sampleprep ID:

SP002066

Sampleprep Summary:

Ribitol was added as an internal standard for normalization, then acetonitrile was added to remove protein. After thoroughly mixing the samples, centrifugation was performed. The upper 1.6 ml was evaporated in a centrifugal concentrator and freeze-dried overnight. Methoxyamine hydrochloride in pyridine and N-methyl-N-trimethylsilyltrifluoroacetamide were added to the samples as derivatizing agents, then they were thoroughly mixed and centrifuged, after which the upper 50 μl of each sample was transferred to a vial.