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MB Sample ID: SA185585

Local Sample ID:	BM71P3
Subject ID:	SU002062
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002068
Sampleprep Summary:	One hundred µl culture media was thawed on ice and then centrifuged at 14,000 x g for 15 min at 4◦C. For each sample, 54 µl of media supernatant was transferred to a new Eppendorf tube. 30 µl of remaining media in each sample from the same cell line was pooled together to generate six 54 µl internal quality control (QC) samples. 6 µl of 10/3 mM DSS-D6 (Cambridge Isotope Laboratory) in D2O (Cambridge Isotope Laboratory) was then added into each sample. In addition, six buffer blanks (6 µl of 10/3 mM DSS-D6 in 54 µl D2O) samples were added in each cell line for quality assurance purpose. We had 10 replicates for each cell line and time point. A total of 262 samples including 250 experimental samples, 6 pooled samples and 6 buffer blanks were generated for the iMSC group. A total of 222 samples including 210 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM71. A total of 242 samples including 230 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM182. All samples were randomized to reduce technical variance.

Sampleprep ID:

SP002068

Sampleprep Summary:

One hundred µl culture media was thawed on ice and then centrifuged at 14,000 x g for 15 min at 4◦C. For each sample, 54 µl of media supernatant was transferred to a new Eppendorf tube. 30 µl of remaining media in each sample from the same cell line was pooled together to generate six 54 µl internal quality control (QC) samples. 6 µl of 10/3 mM DSS-D6 (Cambridge Isotope Laboratory) in D2O (Cambridge Isotope Laboratory) was then added into each sample. In addition, six buffer blanks (6 µl of 10/3 mM DSS-D6 in 54 µl D2O) samples were added in each cell line for quality assurance purpose. We had 10 replicates for each cell line and time point. A total of 262 samples including 250 experimental samples, 6 pooled samples and 6 buffer blanks were generated for the iMSC group. A total of 222 samples including 210 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM71. A total of 242 samples including 230 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM182. All samples were randomized to reduce technical variance.