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MB Sample ID: SA186017
| Local Sample ID: | CatS_Bov3_5 |
| Subject ID: | SU002071 |
| Subject Type: | Insect |
| Subject Species: | Duponchelia fovealis |
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Sample Preparation:
| Sampleprep ID: | SP002077 |
| Sampleprep Summary: | After the direct contact bioassay, the caterpillars were arranged by treatment, then macerated in liquid nitrogen (N2). Extraction was performed with 200 mg of the macerate added to a 1 mL microtube (Eppendorf, Germany) previously treated with methanol. Following this, 125 μL of chloroform (CHCl3), 50 μL ultra-pure water (H2O), and 250 μL cold methanol (CH3OH) were added to the macerate. The microtubes were vigorously vortexed and placed in an ultrasonic bath (Odontobrás, Ribeirão-SP) at 20 Hz and approximately 4ºC for 10 minutes. Then, 50 μL of CHCl3 and 50 μL of H2O were added, and the tubes were vortexed again. The samples were centrifuged (Eppendorf, Germany) for 5 minutes at 14000 rpm and 4°C, and the supernatant was filtered on a Whatman® 0.22 µm filter (Merck, Germany) and transferred to a glass vial. The vial was taken to a lyophilizer (Thermo Fischer Scientific, MA) until the samples had completely dried. Finally, the lyophilized samples were resuspended in 200 µL of extraction solution and aliquoted for use in the GC-MS and LC-MS/MS. |
| Sampleprep Protocol Filename: | Metabolite_Extraction |
| Extract Storage: | -80℃ |
