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MB Sample ID: SA186751

Local Sample ID:	44
Subject ID:	SU002074
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002080
Sampleprep Summary:	Two hundred microliters of frozen supernatant were thawed on ice and evaporated to dryness using a SpeedVac Concentrator System (Thermo Fisher Scientific, Waltham, MA). Then, it was resuspended in 100 µL of 0.2 mM methionine sulfone (MetS) in 0.1 M formic acid. Samples were vortex-mixed for 1 min, transferred to a Millipore filter (30 kDa protein cutoff) and centrifuged for 40 min at 2000 xg at 4 °C. Finally, the ultrafiltrate was transferred to a CE-MS vial for analysis. Quality control samples (QC) were prepared by pooling equal volumes of plasma supernatant from each sample and were treated as previously described. Finally, blank solutions were also prepared with MeOH:EtOH (1:1, v/v).

Sampleprep ID:

SP002080

Sampleprep Summary:

Two hundred microliters of frozen supernatant were thawed on ice and evaporated to dryness using a SpeedVac Concentrator System (Thermo Fisher Scientific, Waltham, MA). Then, it was resuspended in 100 µL of 0.2 mM methionine sulfone (MetS) in 0.1 M formic acid. Samples were vortex-mixed for 1 min, transferred to a Millipore filter (30 kDa protein cutoff) and centrifuged for 40 min at 2000 xg at 4 °C. Finally, the ultrafiltrate was transferred to a CE-MS vial for analysis. Quality control samples (QC) were prepared by pooling equal volumes of plasma supernatant from each sample and were treated as previously described. Finally, blank solutions were also prepared with MeOH:EtOH (1:1, v/v).