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MB Sample ID: SA188218
| Local Sample ID: | 200605_Smp_GBTFate2MT5-pos_A_pos_HILIC |
| Subject ID: | SU002089 |
| Subject Type: | Other organism |
| Subject Species: | Natural mixed marine microbial community |
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Sample Preparation:
| Sampleprep ID: | SP002095 |
| Sampleprep Summary: | Polar and nonpolar metabolites were extracted using a modified Bligh−Dyer extraction using 1:1 methanol/water (aqueous phase) and dichloromethane (organic phase). Methodological blanks were extracted and analyzed along with each sample set. Each sample was aliquotted into two vials and isotope-labeled internal standards were added either before or after the extraction to one vial for all samples, blanks, and pooled samples. To evaluate the effect of obscuring variation due to different matrix strengths and analytical drift, pooled samples were run at both full and half concentration (diluted with water) at least three times throughout a sample set. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Bligh-Dyer |
| Extract Storage: | -80℃ |
