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MB Sample ID: SA189732
Local Sample ID: | 20200810-BE12-Glutamine-4 |
Subject ID: | SU002106 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Genotype Strain: | NF54 attB |
Gender: | Not applicable |
Cell Counts: | 1x10^8 |
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Sample Preparation:
Sampleprep ID: | SP002112 |
Sampleprep Summary: | Once samples were obtained, metabolism was quenched with 90% methanol containing 0.25 uM labeled aspartate, cells were centrifuged, and the supernatant was removed by nitrogen drying. Sample was reconstituted in 3% HPLC-grade methanol and run on the instrument. |
Processing Method: | Wash, spin, quench, spin, dry, store at -80 C until run on the instrument, resuspend |
Processing Storage Conditions: | On ice |
Extraction Method: | 90% methanol extraction |
Extract Cleanup: | Centrifugation and nitrogen drying |
Sample Resuspension: | 100 uL 3% methanol with 1 uM chlorpropamide |
Sample Spiking: | 0.25 uM Labelled Aspartate, 1 uM chlorpropamide |