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MB Sample ID: SA194771

Local Sample ID:	0412_CD27+_Labeled_3
Subject ID:	SU002153
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090
Age Or Age Range:	6-8 weeks old
Weight Or Weight Range:	15-20 grams
Gender:	Male and female
Cell Strain Details:	C57Bl/6

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Sample Preparation:

Sampleprep ID:	SP002159
Sampleprep Summary:	The cells were rinsed in cold PBS and quenched using a mixture containing 2 mL acetonitrile and 1.5 mL H2O. After adding 1 mL chloroform, the sample was homogenized and centrifuged at 3,000 rpm, 4 oC for 20 min. The top layer was transferred to a new tube and lyophilized. The dried sample was then dissolved in 100 µL 20% acetonitrile and vigorously vortex-mixed for 3 min. After centrifugation at 14,000 rpm, 4oC for 20 min, the supernatant was collected for two-dimensional liquid chromatography-mass spectrometry (2DLC-MS) analysis. All samples were analyzed on a Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer coupled with a Thermo DIONEX UltiMate 3000 HPLC system (Thermo Fisher Scientific, Waltham, MA, USA). The UltiMate 3000 HPLC system was equipped with a reversed phase chromatography (RPC) column and a hydrophilic interaction chromatography (HILIC) column. The two columns were configured in parallel 2DLC mode.

Sampleprep ID:

SP002159

Sampleprep Summary:

The cells were rinsed in cold PBS and quenched using a mixture containing 2 mL acetonitrile and 1.5 mL H2O. After adding 1 mL chloroform, the sample was homogenized and centrifuged at 3,000 rpm, 4 oC for 20 min. The top layer was transferred to a new tube and lyophilized. The dried sample was then dissolved in 100 µL 20% acetonitrile and vigorously vortex-mixed for 3 min. After centrifugation at 14,000 rpm, 4oC for 20 min, the supernatant was collected for two-dimensional liquid chromatography-mass spectrometry (2DLC-MS) analysis. All samples were analyzed on a Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer coupled with a Thermo DIONEX UltiMate 3000 HPLC system (Thermo Fisher Scientific, Waltham, MA, USA). The UltiMate 3000 HPLC system was equipped with a reversed phase chromatography (RPC) column and a hydrophilic interaction chromatography (HILIC) column. The two columns were configured in parallel 2DLC mode.