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MB Sample ID: SA194798
Local Sample ID: | SS-001 |
Subject ID: | SU002155 |
Subject Type: | Insect |
Subject Species: | Drosophila melanogaster |
Taxonomy ID: | 7227 |
Age Or Age Range: | 3rd instar larvae |
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Sample Preparation:
Sampleprep ID: | SP002161 |
Sampleprep Summary: | : Frozen larvae were homogenized in 1 ml cold (-80 °C) 80% methanol on dry ice using a probe homogenizer. Samples were clarified by centrifugation for 5 min at 16,000 x gmax at 4 °C and the supernatants were transferred to new Eppendorf tubes. The remaining pellets were resuspended in RIPA lysis buffer and total protein content was determined by the BCA method. A volume of the supernatant equivalent to 5 µg of protein was transferred to a glass vial, dried using an EZ-2 Elite evaporator (SP Scientific), and stored at -80 °C until analysis by LC-MS. |