Return to study ST002076 main page
MB Sample ID: SA196289
Local Sample ID: | AI_3 |
Subject ID: | SU002159 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Genotype Strain: | K-12 MG1655 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002165 |
Sampleprep Summary: | Samples were extracted from the Wildtype, FA, AI and FI mutants grown in a bioreactor anaerobically in glucose minimal media at 37 degrees Celsius and 150 rpm. The metabolites were extracted using the Methanol-Chloroform-Water method. The samples were spiked with an equal volume of 13C-labelled internal standard taken from the same batch at an earlier stage to prevent to account for losses due to metabolite degradation and to enable robust quantification circumventing the ion-suppression effects. |
Sampleprep Protocol Filename: | Method_msi.pdf |