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MB Sample ID: SA196302
| Local Sample ID: | FI_2 |
| Subject ID: | SU002159 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Genotype Strain: | K-12 MG1655 |
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Sample Preparation:
| Sampleprep ID: | SP002165 |
| Sampleprep Summary: | Samples were extracted from the Wildtype, FA, AI and FI mutants grown in a bioreactor anaerobically in glucose minimal media at 37 degrees Celsius and 150 rpm. The metabolites were extracted using the Methanol-Chloroform-Water method. The samples were spiked with an equal volume of 13C-labelled internal standard taken from the same batch at an earlier stage to prevent to account for losses due to metabolite degradation and to enable robust quantification circumventing the ion-suppression effects. |
| Sampleprep Protocol Filename: | Method_msi.pdf |
