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MB Sample ID: SA201214
| Local Sample ID: | A549 Non-infection 3 |
| Subject ID: | SU002181 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | American Type Culture Collection |
| Cell Strain Details: | A549 (tissue, lung cancer; gender, male), LS174T (tissue, colon cancer; gender, female) |
| Cell Counts: | 10,000,000 |
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Sample Preparation:
| Sampleprep ID: | SP002187 |
| Sampleprep Summary: | Total lipids were extracted from samples using the Bligh-Dyer method. An aliquot of the organic phase was added to an equal volume of methanol before being loaded onto a DEAE-cellulose column (Wako Chemical) pre-equilibrated with chloroform. After successive washes with chloroform/methanol (1:1, v/v), acidic phospholipids were eluted with chloroform/methanol/HCl/water (12:12:1:1, v/v), followed by evaporation to dryness to yield a residue was soluble in methanol. |
| Extraction Method: | the Bligh-Dyer method |
