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MB Sample ID: SA204243
Local Sample ID: | G-4 |
Subject ID: | SU002206 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP002212 |
Sampleprep Summary: | 100-130 mg tissue samples tumor tissues have to be broken by oscillation with 60HZ and 2 minutes in iced 80% methanol solution (mixed with internal standard 0.001mg/ml 4-chloro-DL-phenylalanine). centrifuging at 20,000 g for 10 min at 4 °C, and mixing the supernatants with 800 μL ice-cold acetonitrile. Then the supernatant obtained by centrifugation was filtered through a 0.22 µm organic phase membrane, blown dry in nitrogen, reconstituted in 100 µL of water and transferred to vials for metabolomics assay. |