Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA205264

Local Sample ID:	C8_9
Subject ID:	SU002222
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP002228
Sampleprep Summary:	An extraction solvent consisting of a mixture of isotopically labeled internal standards including 808 µM 13C6 arginine and 212 µM 13C methionine D3 was added to methanol in a 1:60 ratio and stored at 4 °C until further use. Serum samples were thawed on ice, followed by protein precipitation in a 10 µl serum aliquot with extraction solvent in a 1:3 ratio. Samples were vortexed for 30 s and centrifuged at 13,000 rpm for 7 min. To enhance CE peak shape, the resulting supernatant was diluted in a 1:4 ratio with the sample diluent containing 133 mM ammonium acetate, 0.1% formic acid and 1 µM 13C phenylalanine.

Sampleprep ID:

SP002228

Sampleprep Summary:

An extraction solvent consisting of a mixture of isotopically labeled internal standards including 808 µM 13C6 arginine and 212 µM 13C methionine D3 was added to methanol in a 1:60 ratio and stored at 4 °C until further use. Serum samples were thawed on ice, followed by protein precipitation in a 10 µl serum aliquot with extraction solvent in a 1:3 ratio. Samples were vortexed for 30 s and centrifuged at 13,000 rpm for 7 min. To enhance CE peak shape, the resulting supernatant was diluted in a 1:4 ratio with the sample diluent containing 133 mM ammonium acetate, 0.1% formic acid and 1 µM 13C phenylalanine.