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MB Sample ID: SA205273
Local Sample ID: | C13_10 |
Subject ID: | SU002222 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP002228 |
Sampleprep Summary: | An extraction solvent consisting of a mixture of isotopically labeled internal standards including 808 µM 13C6 arginine and 212 µM 13C methionine D3 was added to methanol in a 1:60 ratio and stored at 4 °C until further use. Serum samples were thawed on ice, followed by protein precipitation in a 10 µl serum aliquot with extraction solvent in a 1:3 ratio. Samples were vortexed for 30 s and centrifuged at 13,000 rpm for 7 min. To enhance CE peak shape, the resulting supernatant was diluted in a 1:4 ratio with the sample diluent containing 133 mM ammonium acetate, 0.1% formic acid and 1 µM 13C phenylalanine. |