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MB Sample ID: SA206365
Local Sample ID: | WT-1 |
Subject ID: | SU002239 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | AppNL-G-F or wild type mice |
Age Or Age Range: | 7 months old |
Gender: | Male and female |
Animal Animal Supplier: | RIKEN Center for Brain Science |
Animal Light Cycle: | 12 hours light and dark cycle |
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Sample Preparation:
Sampleprep ID: | SP002245 |
Sampleprep Summary: | For CE-FTMS, 40 µL of plasma was added to 160 µL of methanol containing internal standards at 0°C, 120 µL of Milli-Q water was added and mixed thoroughly, and 240 µL of this mixture was added to a Millipore 5 kDa cutoff filter (ULTRAFREE MC PLHCC, HMT) at 9100 × g for 120 min at 4°C to remove macromolecules. The filtrate was evaporated to dryness under vacuum, dissolved in 40 µL Milli-Q water, and donated to HMT's ω Scan package using capillary electrophoresis Fourier transform mass spectrometry (CE-FTMS).For LC-TOFMS, 80 µL of plasma was added to 240 µL of 1% formic acid/acetonitrile containing internal standards at 0ºC. The mixture was centrifuged at 2,300 x g, 4ºC for 5 min, then centrifuged at 2,300 x g, 4ºC for 5 min, followed by centrifugation of a hybrid SPE phospholipid cartridge (30 mg/mL of hybrid SPE-phospholipid, 30 mg/mL of (SUPELCO) and filtered to remove phospholipids. The filtrate was then evaporated to dryness under nitrogen, dissolved in 80 μL of 50% isopropanol (v/v), and metabolomic analysis was performed using liquid chromatography time-of-flight mass spectrometry (LC-TOFMS). |