Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA206384

Local Sample ID:	PBS6-5
Subject ID:	SU002240
Subject Type:	Fish
Subject Species:	Epinephelus coioides

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Sample Preparation:

Sampleprep ID:	SP002246
Sampleprep Summary:	The deep-frozen spleen tissue in each replicate was accurately weighed (about 15 mg) and then transferred to an Eppendorf tube. Each sample was first spiked with 300 μL of methanol containing internal standards and then homogenized at 65 Hz for 2.5 min. The homogenate was mixed with 1 mL of MTBE, and thoroughly vortexed for 30 min. Then, 300 μL of Milli-Q water was added to the tube, followed by vortexing for 1 min and centrifugation for 10 min to form a two-phase system (12,000 rpm, 4 ℃). Each specimen was prepared by mixing 250 μL of the top layer plus 150 μL of the bottom layer accurately and then vacuum-dried in a Speedvac concentrator (Thermo Scientific, USA).

Sampleprep ID:

SP002246

Sampleprep Summary:

The deep-frozen spleen tissue in each replicate was accurately weighed (about 15 mg) and then transferred to an Eppendorf tube. Each sample was first spiked with 300 μL of methanol containing internal standards and then homogenized at 65 Hz for 2.5 min. The homogenate was mixed with 1 mL of MTBE, and thoroughly vortexed for 30 min. Then, 300 μL of Milli-Q water was added to the tube, followed by vortexing for 1 min and centrifugation for 10 min to form a two-phase system (12,000 rpm, 4 ℃). Each specimen was prepared by mixing 250 μL of the top layer plus 150 μL of the bottom layer accurately and then vacuum-dried in a Speedvac concentrator (Thermo Scientific, USA).