Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA208946

Local Sample ID:	CR1-02_2_1_729
Subject ID:	SU002259
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Cell Strain Details:	MCF-7

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Sample Preparation:

Sampleprep ID:	SP002265
Sampleprep Summary:	Around 2x106 MCF-7 cells were plated per flask and then separately treated with SIMR3058 or SIMR3066 compounds for 12 hrs to perform proteomic analysis; the uniform number of cells per flask for each sample was to avoid the effect of variable cell number on the outcome of the specific experiment. A volume of 1 mL of the extraction solvent (methanol + 0.1% formic acid) was added to the cells, which quenched cellular metabolic activity. The cells were vortexed for 2 min to ensure the quantitative extraction of metabolites and stored in ice for 1 hr. After this, the insoluble cell matrices were subjected to intermittent ultrasonication using the COPLEY sonicator (QSONICA SONICATOR, USA) under 30% amplifier and for 30 seconds with an ice bath employed throughout the process. Following that, cells debris were centrifuged (15000 rpm, 10 min, -4 °C), and the supernatants were collected and processed for proteomics and metabolomics analysis.

Sampleprep ID:

SP002265

Sampleprep Summary:

Around 2x106 MCF-7 cells were plated per flask and then separately treated with SIMR3058 or SIMR3066 compounds for 12 hrs to perform proteomic analysis; the uniform number of cells per flask for each sample was to avoid the effect of variable cell number on the outcome of the specific experiment. A volume of 1 mL of the extraction solvent (methanol + 0.1% formic acid) was added to the cells, which quenched cellular metabolic activity. The cells were vortexed for 2 min to ensure the quantitative extraction of metabolites and stored in ice for 1 hr. After this, the insoluble cell matrices were subjected to intermittent ultrasonication using the COPLEY sonicator (QSONICA SONICATOR, USA) under 30% amplifier and for 30 seconds with an ice bath employed throughout the process. Following that, cells debris were centrifuged (15000 rpm, 10 min, -4 °C), and the supernatants were collected and processed for proteomics and metabolomics analysis.