Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA210825

Local Sample ID:	H-101
Subject ID:	SU002286
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002292
Sampleprep Summary:	Metabolite extraction: Samples were thawed on ice and 20 μL of sample was diluted in 480 μL of LC-MS grade cold methanol/acetonitrile/water (5:3:2). Samples were incubated at 4°C for 30 min with vigorous vortexing. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 50 uL aliquots were transferred to autosampler vials. Lipid extraction: Samples were thawed on ice and 10 uL of sample was diluted in 90 uL of LC-MS grade cold methanol. Samples were briefly vortexed to mix then incubated for 30 min at -20C. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 25 uL aliquots were transferred to autosampler vials and diluted with 25 uL of 10 mM ammonium acetate.

Sampleprep ID:

SP002292

Sampleprep Summary:

Metabolite extraction: Samples were thawed on ice and 20 μL of sample was diluted in 480 μL of LC-MS grade cold methanol/acetonitrile/water (5:3:2). Samples were incubated at 4°C for 30 min with vigorous vortexing. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 50 uL aliquots were transferred to autosampler vials. Lipid extraction: Samples were thawed on ice and 10 uL of sample was diluted in 90 uL of LC-MS grade cold methanol. Samples were briefly vortexed to mix then incubated for 30 min at -20C. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 25 uL aliquots were transferred to autosampler vials and diluted with 25 uL of 10 mM ammonium acetate.