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MB Sample ID: SA211120
Local Sample ID: | H-119 |
Subject ID: | SU002286 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002292 |
Sampleprep Summary: | Metabolite extraction: Samples were thawed on ice and 20 μL of sample was diluted in 480 μL of LC-MS grade cold methanol/acetonitrile/water (5:3:2). Samples were incubated at 4°C for 30 min with vigorous vortexing. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 50 uL aliquots were transferred to autosampler vials. Lipid extraction: Samples were thawed on ice and 10 uL of sample was diluted in 90 uL of LC-MS grade cold methanol. Samples were briefly vortexed to mix then incubated for 30 min at -20C. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 25 uL aliquots were transferred to autosampler vials and diluted with 25 uL of 10 mM ammonium acetate. |