Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA211361

Local Sample ID:	human_tumor_CD73
Subject ID:	SU002289
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP002295
Sampleprep Summary:	As stated in the manuscript Frozen resection tissue from 9 glioblastomas, including 4 cases with high CD73 and 5 cases with low CD73 by IHC, were selected for MALDI MSI. Tissue was sectioned to 10 µm, thaw mounted onto indium-tin-oxide (ITO) slides, and serial sections were obtained for H&E staining. A high-resolution image of the whole H&E tissues was obtained through image stitching (Zeiss Observer Z.1, Oberkochen, Germany) using a plan-apochromat lens (20×) using an AxioCam MR3 camera. Matrix preparation of 1,5-diaminonaphthalene hydrochloride was prepared to a concentration of 4.3 mg/mL in 4.5/5/0.5 HPLC grade water/ethanol/1 M HCl (v/v/v). The matrix was sprayed using a TM-sprayer (HTX imaging, Carrboro, NC) with parameters of a flow rate (0.09 mL/min), spray nozzle velocity (1200 mm/min), spray nozzle temperature (75°C), nitrogen gas pressure (10 psi), track spacing (2 mm) and a four pass spray.

Sampleprep ID:

SP002295

Sampleprep Summary:

As stated in the manuscript Frozen resection tissue from 9 glioblastomas, including 4 cases with high CD73 and 5 cases with low CD73 by IHC, were selected for MALDI MSI. Tissue was sectioned to 10 µm, thaw mounted onto indium-tin-oxide (ITO) slides, and serial sections were obtained for H&E staining. A high-resolution image of the whole H&E tissues was obtained through image stitching (Zeiss Observer Z.1, Oberkochen, Germany) using a plan-apochromat lens (20×) using an AxioCam MR3 camera. Matrix preparation of 1,5-diaminonaphthalene hydrochloride was prepared to a concentration of 4.3 mg/mL in 4.5/5/0.5 HPLC grade water/ethanol/1 M HCl (v/v/v). The matrix was sprayed using a TM-sprayer (HTX imaging, Carrboro, NC) with parameters of a flow rate (0.09 mL/min), spray nozzle velocity (1200 mm/min), spray nozzle temperature (75°C), nitrogen gas pressure (10 psi), track spacing (2 mm) and a four pass spray.