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MB Sample ID: SA211480
| Local Sample ID: | Maryam_2nd-set_Urine_DC_Vaccine_D1_D8 |
| Subject ID: | SU002294 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Gender: | Not applicable |
| Animal Animal Supplier: | Jackson Labs (Bar Harbor, ME) |
| Animal Housing: | Housed in a temperature of 22 oC |
| Animal Light Cycle: | 12-hour light/12-hour dark |
| Animal Water: | free access to food and water (3-5 animals per cage). |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002300 |
| Sampleprep Summary: | No filtration was performed for urine samples. The urine samples were centrifuged at 4 oC, with 13.2 K rpm speed before mixing it with an internal reference and phosphate buffer system. |
| Sampleprep Protocol Filename: | DC_Vaccine_treated_NMR_urine_Procedures.docx |
| Processing Method: | None |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | None |
| Extract Storage: | -80℃ |
| Sample Resuspension: | In 500 microliter of 150 mM phosphate buffer (pH 7.2) with 2 mM EDTA, 0.5 mM DSS and 0.2% sodium azide for aqueous phase samples. |
| Sample Spiking: | 0.5 mM DSS for urine samples |
