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MB Sample ID: SA216335
| Local Sample ID: | Plasma05-02_10_1_2841 |
| Subject ID: | SU002336 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 39.48 +-10.0 |
| Weight Or Weight Range: | 29.09 +-4.76 |
| Gender: | Male and female |
| Human Inclusion Criteria: | Overweight (BMI>=25), otherwise healthy, between 18 and 60 years, no chronic disease, willingness to observe Ramadan Fasting |
| Human Exclusion Criteria: | Medication use within 1 week, bariatric surgery within 6 months, or any weight reduction regimen within 1 month of the commencement of Ramadan |
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Sample Preparation:
| Sampleprep ID: | SP002342 |
| Sampleprep Summary: | For analysis, an aliquot of thawed plasma sample was transferred into a microcentrifuge tube and cold methanol added at 3:1 v/v (i.e., 30 μL sample, add 90 μL cold methanol), vortexed and allowed to sit at –20ºC for two hours. Next, samples were centrifuged at 20,817 x g for 15 min at 4ºC and the supernatant transferred to a new microcentrifuge tube. Usually, the transferred volume was three times that of the original sample (i.e., for a 30 μL sample, add 90 μL cold methanol, then transfer 90 μL supernatant). The samples were dried using a Speed vac at 30 – 40°C and the dried sample stored in a –80ºC freezer for further use or dissolved in solvent for LC-MS/MS analysis. Samples were resuspended in the starting solvent (0.1% formic acid) where the volume was three times the original plasma volume, i.e., 90 μL for 30 μL serum/plasma aliquot. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | 4℃ |
