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MB Sample ID: SA217485
| Local Sample ID: | SH1 2 |
| Subject ID: | SU002351 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP002357 |
| Sampleprep Summary: | nuclear magnetic resonance (NMR) analysis on thawed saliva aliquots (500 l, n=3 per participant) was performed using a previously published method 11. Briefly, samples were centrifuged for 5 minutes at 13,000 RPM to remove cell debris. The supernatant was removed for NMR analysis and mixed at a ratio of 4:1 with sodium trimethylsilyl propionate (TSP) buffer and added to a 5mm NMR tube (Bruker, Coventry, UK). Spectra were acquired on a 600 MHz NMR spectrophotometer (Bruker). The spectra were processed on TopSpin (Bruker) to correct the phase and baseline, and to calibrate the TSP peak to 0 ppm. The identity of metabolites were assigned and the concentration (mM) obtained from spectra using Chenomx NMR Suite version 8.5 (Chenomix Ltd, Canada). |
| Processing Storage Conditions: | On ice |
