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MB Sample ID: SA217579
| Local Sample ID: | H_C_256 |
| Subject ID: | SU002355 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 8.7-18.6 (year) |
| Gender: | Male and female |
| Human Ethnicity: | Korean |
| Human Inclusion Criteria: | We recruited children and adolescent participants who visited the pediatric departments of the Hallym University Sacred Heart Hospital and Seoul National University Children’s Hospital from January 2019 to May 2020, after obtaining informed consent from the children and their parents. |
| Human Exclusion Criteria: | We excluded participants who were taking alcohol or medications known to affect the results of liver function tests. Participants with viral hepatitis, such as hepatitis A, B, or C, or with Epstein–Barr virus, Wilson’s disease, autoimmune hepatitis, or muscular disease were also excluded. |
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Sample Preparation:
| Sampleprep ID: | SP002361 |
| Sampleprep Summary: | frozen plasma samples were thawed on ice and vortexed, followed by centrifugation at 2,750 × g, 4℃ for 5 min before the samples were loaded onto a 96-well plate with a filter. After the addition of 10 μL of analytical and pooled plasma samples and calibration standards to each well, the plates were dried with a nitrogen evaporator and derivatized with phenyl isothiocyanate. Then, dried samples were extracted with ammonium acetate solution in methanol and aliquoted into two deep-well plates for liquid chromatography mode and flow injection analysis (FIA) mode (described in the manual), followed by dilution with water and an FIA solvent, respectively. Both deep-well plates were placed in an autosampler of Ultimate 3000 ultra-performance liquid chromatography coupled with a Q Exactive Plus hybrid quadrupole-orbitrap mass spectrometer (Thermo Fisher Scientific, MA, USA) and analyzed using the validated method. |
| Sampleprep Protocol Filename: | UM-p400-HR-Thermo-3.pdf |
| Processing Storage Conditions: | On ice |
| Extract Storage: | 4℃ |
