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MB Sample ID: SA217808
| Local Sample ID: | 4710-C-R2 |
| Subject ID: | SU002358 |
| Subject Type: | Plant |
| Subject Species: | Hordeum vulgare |
| Taxonomy ID: | 4513 |
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Sample Preparation:
| Sampleprep ID: | SP002364 |
| Sampleprep Summary: | For metabolite profiling, three biological replicates of 12 mg of barley embryos manually dissected from dry mature seeds were ground with mortar and pestle in liquid nitrogen and stored at -80 °C. All steps were performed in 2 ml Safelock Eppendorf tubes. The ground frozen samples (12 mg) were resuspended in 1 ml of frozen (-20°C) Water:Acetonitrile:Isopropanol (2:3:3 v/v/v/) containing Ribitol at 4 mg.L-1 and extracted for 10 min at 4°C with shaking at 1400 rpm in an Eppendorf Thermomixer. Insoluble material was removed by centrifugation at 20000g for 5 min. 25 µL were collected and dried for 150 min in a SpeedVac. Fiehn et al (the Plant Journal (2008) 53, 691-704). |
| Processing Storage Conditions: | -20℃ |
| Extract Storage: | -80℃ |
