Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA219161

Local Sample ID:	SN_GP61_1_2
Subject ID:	SU002373
Subject Type:	Bacteria
Subject Species:	Escherichia coli
Taxonomy ID:	562

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Sample Preparation:

Sampleprep ID:	SP002379
Sampleprep Summary:	Metabolites from supernatant were extracted in 100% methanol, precipitated at -20°C for 1 hour, centrifuged and diluted into a linear range (1:20 final dilution) with 50% methanol. To extract intracellular metabolites, bacterial pellet was snap-frozen in liquid nitrogen, resuspended in cell lysis buffer (1M Tris, 5M NaCL, 0.5M EDTA, 10% SDS in H2O) and sonicated. Metabolites were extracted as described above

Sampleprep ID:

SP002379

Sampleprep Summary:

Metabolites from supernatant were extracted in 100% methanol, precipitated at -20°C for 1 hour, centrifuged and diluted into a linear range (1:20 final dilution) with 50% methanol. To extract intracellular metabolites, bacterial pellet was snap-frozen in liquid nitrogen, resuspended in cell lysis buffer (1M Tris, 5M NaCL, 0.5M EDTA, 10% SDS in H2O) and sonicated. Metabolites were extracted as described above