Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA226504

Local Sample ID:	Plasma69-02_75_1_2427
Subject ID:	SU002387
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002393
Sampleprep Summary:	Plasma was obtained after the collection of samples into heparinized tubes followed by centrifugation for 5 minutes (3000g). The samples were stored at –80 ºC for long-term storage until further metabolomics analysis. An aliquot of plasma sample into a microcentrifuge tube and add cold methanol into the sample at 3:1 v/v (i.e., 30 μL sample, add 90 μL cold methanol) vortex and allow to sit in –20ºC for two hrs. Next, centrifuge the samples at 20,817 x g for 15 min at 4ºC. Then, transfer the supernatant to a new microcentrifuge tube. Usually, transfer three times the original sample volume (i.e., for 30 μL sample, add 90 μL cold methanol, then transfer 90 μL supernatant). Dry down the sample using Speed vac at 30 – 40°C. Store the dried sample in a –80ºC freezer for further use or dissolve it in solvent for LC-MS/MS analysis

Sampleprep ID:

SP002393

Sampleprep Summary:

Plasma was obtained after the collection of samples into heparinized tubes followed by centrifugation for 5 minutes (3000g). The samples were stored at –80 ºC for long-term storage until further metabolomics analysis. An aliquot of plasma sample into a microcentrifuge tube and add cold methanol into the sample at 3:1 v/v (i.e., 30 μL sample, add 90 μL cold methanol) vortex and allow to sit in –20ºC for two hrs. Next, centrifuge the samples at 20,817 x g for 15 min at 4ºC. Then, transfer the supernatant to a new microcentrifuge tube. Usually, transfer three times the original sample volume (i.e., for 30 μL sample, add 90 μL cold methanol, then transfer 90 μL supernatant). Dry down the sample using Speed vac at 30 – 40°C. Store the dried sample in a –80ºC freezer for further use or dissolve it in solvent for LC-MS/MS analysis