Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA226677

Local Sample ID:	46
Subject ID:	SU002390
Subject Type:	Mammal
Subject Species:	Myotis lucifugus

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Sample Preparation:

Sampleprep ID:	SP002396
Sampleprep Summary:	Tissue samples were homogenized in 300 μL of extraction buffer (isopropanol [IPA]) containing internal standards for the above lipid classes per the manufacturer’s instructions. A 10 μL aliquot of the tissue/extraction buffer mixture was collected for determining protein concentration. The samples were vortexed for 30 seconds, incubated on ice for 30 min, and then centrifuged for 10 min (10,000 x g, 4°C). The supernatant was transferred to a MS vial for LC-MS analysis. A pooled sample of all aliquots was prepared as a quality control and run every 10 samples in addition to the NIST SRM 1950 plasma mix. The NIST SRM 1950 plasma was prepared by aliquoting 20 µL in 100 μL of chilled IPA containing internal standards. Samples were vortexed for 1 min, incubated on ice for 30 min then incubated at -20°C for 2 hours for complete protein precipitation, then centrifuged for 20 min (10,000 x g, 4°C).

Sampleprep ID:

SP002396

Sampleprep Summary:

Tissue samples were homogenized in 300 μL of extraction buffer (isopropanol [IPA]) containing internal standards for the above lipid classes per the manufacturer’s instructions. A 10 μL aliquot of the tissue/extraction buffer mixture was collected for determining protein concentration. The samples were vortexed for 30 seconds, incubated on ice for 30 min, and then centrifuged for 10 min (10,000 x g, 4°C). The supernatant was transferred to a MS vial for LC-MS analysis. A pooled sample of all aliquots was prepared as a quality control and run every 10 samples in addition to the NIST SRM 1950 plasma mix. The NIST SRM 1950 plasma was prepared by aliquoting 20 µL in 100 μL of chilled IPA containing internal standards. Samples were vortexed for 1 min, incubated on ice for 30 min then incubated at -20°C for 2 hours for complete protein precipitation, then centrifuged for 20 min (10,000 x g, 4°C).