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MB Sample ID: SA227009
| Local Sample ID: | N3 |
| Subject ID: | SU002396 |
| Subject Type: | Bird |
| Subject Species: | Gallus gallus |
| Taxonomy ID: | 9031 |
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Sample Preparation:
| Sampleprep ID: | SP002402 |
| Sampleprep Summary: | Cecal contents were lyophilized and homogenated. Then, 10 mg of the sample were mixed with 0.75mL of cold 75% (v/v) methanol, 0.1% (v/v) formic acid, spiked with 10μg/ml formononetin as internal standard. Then, the mix were shaked for 40’ at 20Hz using a Mixer Mill 300 (Qiagen) and centrifuged at 20,000 xg for 15 min at 4°C. The suspension (600μL) was transferred to a new 2-mL conical tube. |
