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MB Sample ID: SA237020
Local Sample ID: | Ctrl_1_RP_Pos |
Subject ID: | SU002450 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP002456 |
Sampleprep Summary: | Cells cultured in 6-well plates were quickly washed once with ice cold 5 mM HEPES, then immediately quenched in 150ul/well ice cold quench buffer (20% methanol, 3 mM sodium fluoride, 0.1% formic acid, 1mM phenylalanine and 100uM EDTA). Each well was harvested using a cell lifter and immediately transferred to a pre-chilled 96-well plate on dry ice. Once completely frozen, cell lysates were lyophilized and stored in the -80oC freezer until the sample run. Lyophilized samples were prepared by resuspending in 50 µL water with D4-taurine (50 µM) as an internal standard and 5 µL of the supernatant was injected for each analysis mode using high performance liquid chromatography (HPLC) into the 6600 Triple TOF LC-MS/MS mass spectrometer (Sciex). |