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MB Sample ID: SA237256
| Local Sample ID: | S_5 |
| Subject ID: | SU002457 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
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Sample Preparation:
| Sampleprep ID: | SP002463 |
| Sampleprep Summary: | Seminal plasma samples (500 µL each) were shipped from Dr. Pilsner’s lab at the University of Massachusetts Amherst to the NC HHEAR Hub with dry ice and stored at -80 °C until analysis. NIST blood plasma aliquots (SRM 1950, 50 µL) were provided by the NC HHEAR hub and used external reference materials. All samples (including study samples and NIST reference materials) were thawed at 4 °C overnight. The seminal plasma sample (50 µL) was transferred from the shipped original tubes to a new set of pre-labeled Lo-Bind Eppendorf tubes for sample preparation. A quality control total pool was made by pooling an additional 7-µL seminal plasma from each of the original samples into a new Lo-Bind Eppendorf tube and then distributed into multiple aliquots within 50 µL each to use as quality control study pools (QCSPs) ran throughout the whole study. LC-MS grade water aliquots (50 µL) were used as blanks. All samples, including study samples, QCSPs, NIST reference material samples, and blanks, were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 (internal standard) and vortexed by the multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. The supernatants (350 µL) were transferred into pre-labeled 2.0 mL Lo-bind Eppendorf tubes and then dried by a SpeedVac overnight. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts, and the samples were thoroughly mixed on the multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by the instrument. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | Described in summary |
