Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA237390

Local Sample ID:	KO2
Subject ID:	SU002466
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	C57BL/6N
Age Or Age Range:	6-weeks old

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP002472
Sampleprep Summary:	Tissues with internal standards were homogenized with ice-cold methanol and extracted by the Bligh–Dyer method using chloroform and methanol to separate and recover the water-soluble fractions. The supernatant was filtered through a 5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies) at 9,100 × g for 2 h at 4 °C, dried in a centrifugal evaporator at 1,500 rpm at 1,000 Pa, and resuspended in 50 μl of ultrapure water before metabolome analysis.

Sampleprep ID:

SP002472

Sampleprep Summary:

Tissues with internal standards were homogenized with ice-cold methanol and extracted by the Bligh–Dyer method using chloroform and methanol to separate and recover the water-soluble fractions. The supernatant was filtered through a 5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies) at 9,100 × g for 2 h at 4 °C, dried in a centrifugal evaporator at 1,500 rpm at 1,000 Pa, and resuspended in 50 μl of ultrapure water before metabolome analysis.