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MB Sample ID: SA238140
Local Sample ID: | iC99-R1-007_3_Gln |
Subject ID: | SU002478 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Cell Biosource Or Supplier: | ForIPS consortium |
Cell Strain Details: | Midbrain neuronal precursor cells differentiated from patient derived induced pluripotent stem cells |
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Sample Preparation:
Sampleprep ID: | SP002484 |
Sampleprep Summary: | Subsequently, cell culture supernatant was stored for profiling the extracellular metabolome. Three replicates per cell line and blanks were washed with 0.9% NaCl and quenched with ice-cold methanol and ice-cold ddH2O (containing 1 µg/ml D6-glutaric acid as internal standard). Cells were scraped and extracts were added into tubes containing ice-cold chloroform. Following vortexing at 1,400 rpm for 20 min at 4°C and centrifugation at 17,000 g for 5 min at 4°C, 300 µl of the polar phase were transferred into GC glass vials with microinsert and dried under vacuum at 4°C. Dried extracts were derivatized using equal amounts of methoxylamine (20 mg/ml in pyridine) and MTBSTFA. |