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MB Sample ID: SA241946
Local Sample ID: | F2-27-03 |
Subject ID: | SU002502 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
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Sample Preparation:
Sampleprep ID: | SP002508 |
Sampleprep Summary: | Lipids were extracted from frozen cell pellets using LC-MS grade cold methanol at a concentration of 2e6 cells/mL. Samples were briefly vortexed to mix then incubated for 30 min at -20C. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 25 uL aliquots were transferred to autosampler vials. |