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MB Sample ID: SA242363
Local Sample ID: | 20191230_neg_rep2_WT_a |
Subject ID: | SU002510 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | HEK293T |
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Sample Preparation:
Sampleprep ID: | SP002516 |
Sampleprep Summary: | Lipids were isolated from collected cultured cells. Cells were washed with PBS, treated with cold 50% methanol (1mL) and transferred to glass vials. Next, chloroform (0.5mL) was added and samples were gently vortexed and centrifuged at 1,000x g for 5 min at 4˚C. Lipids were transferred to a clean glass vial using a glass Hamilton syringe. Lipids were extracted twice using chloroform prior to being dried under nitrogen gas. Samples were normalized according to protein concentration when resuspended in a 1:1:1 solution of methanol:chloroform:isopropanol prior to mass spectrometry (MS) analysis. |