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MB Sample ID: SA242829
Local Sample ID: | DE52 |
Subject ID: | SU002517 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 18-65 |
Gender: | Male and female |
Human Exclusion Criteria: | HIV patients, respiratory infections beside TB, diabetes, chronic renal failure history and transplanted. individuals |
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Sample Preparation:
Sampleprep ID: | SP002523 |
Sampleprep Summary: | Serum samples (100 µL) were extracted with 300 µL of methanol overnight at 4°C. The morning after, the samples were centrifuged for 5 min at 16.000 ×g and 4°C. The supernatant was transferred to a new tube, evaporated in the speedvac, and the pellet was stored at −20°C until further analysis. Previous to LC-MS/MS analysis the pellets were resuspended in 99.16 µL of H2O 0.1%FA. The Internal Standard (IS) – 3-Nitro-L-tyrosine – was added to a final concentration of 42 µM. In addition, four pools of samples were used for metabolite identification. |