Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA244720

Local Sample ID:	PA CP27_2_25_1_973
Subject ID:	SU002536
Subject Type:	Bacteria
Subject Species:	Pseudomonas aeruginosa

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Sample Preparation:

Sampleprep ID:	SP002542
Sampleprep Summary:	A volume of 1 mL of the extraction solvent (methanol +0.1% formic acid) was added to the cell extract. The cell extract was then vortexed for 2 min to ensure the quantitative extraction of the metabolites and stored on ice for 1 h, during which the samples were vortexed every 15 min. After this, the insoluble cell matrices were collected and transferred to centrifuge tubes, intermittent ultrasonication using the COPLEY sonicator (QSONICA SONICATOR, USA) under 30% amplifier and for 30 seconds with an ice bath employed throughout the process. Following that, cells debris were then centrifuged (15000 rpm, 10 minutes, - 4 °C) and the sample supernatants were collected and transferred to LC vials for drying in the EZ-2 Plus (GeneVac-Ipswich, UK) at 37 ± 1 °C. Dried samples were resuspended with 200 µL (water + 0.1% formic acid), and vortexed for 2 min. Finally, the samples were filtered using hydrophilic Nylon Syringe Filter of 0.45µm pore size and analyzed by Q-TOF MS.

Sampleprep ID:

SP002542

Sampleprep Summary:

A volume of 1 mL of the extraction solvent (methanol +0.1% formic acid) was added to the cell extract. The cell extract was then vortexed for 2 min to ensure the quantitative extraction of the metabolites and stored on ice for 1 h, during which the samples were vortexed every 15 min. After this, the insoluble cell matrices were collected and transferred to centrifuge tubes, intermittent ultrasonication using the COPLEY sonicator (QSONICA SONICATOR, USA) under 30% amplifier and for 30 seconds with an ice bath employed throughout the process. Following that, cells debris were then centrifuged (15000 rpm, 10 minutes, - 4 °C) and the sample supernatants were collected and transferred to LC vials for drying in the EZ-2 Plus (GeneVac-Ipswich, UK) at 37 ± 1 °C. Dried samples were resuspended with 200 µL (water + 0.1% formic acid), and vortexed for 2 min. Finally, the samples were filtered using hydrophilic Nylon Syringe Filter of 0.45µm pore size and analyzed by Q-TOF MS.