Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA245471

Local Sample ID:	C762_Thalamus
Subject ID:	SU002542
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	APOE-targeted replacement mice homozygous for human E3 (B6.129P2-Apoe^tm2(APOE3)Mae N8, Taconic #1548-F) or human E4 (B6.129P2- Apoe^tm3(APOE4)Mae N8, Taconic #1549-F) alleles and crossed to the 5x Familial Alzheimer's Disease (5XFAD) model (MMRRC #34840, B6SJL^Tg( APPSwFlLon,PSEN1M146LL286V)6799Vas/Mmjax))
Age Or Age Range:	3 months, 12 months, 24 months
Gender:	Female

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Sample Preparation:

Sampleprep ID:	SP002548
Sampleprep Summary:	Prepared brain hemispheres were cryosectioned to 10 μm thick coronal sections at approximately Bregma -2.00 mm. Brain sections were mounted on glass slides and prepared for MALDI MSI as follows: After desiccation for one hour, slides were sprayed with 14 passes of 7mg/mL N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDC) matrix (Sigma) in 70% methanol (HPLC-grade, Sigma) which was applied at 0.06mL/min with a 3mm offset and a velocity of 1200mm/min at 30°C and 10psi using the M5 Sprayer with a heated tray of 50°C. Slides were used immediately or stored in a desiccator until analysis.

Sampleprep ID:

SP002548

Sampleprep Summary:

Prepared brain hemispheres were cryosectioned to 10 μm thick coronal sections at approximately Bregma -2.00 mm. Brain sections were mounted on glass slides and prepared for MALDI MSI as follows: After desiccation for one hour, slides were sprayed with 14 passes of 7mg/mL N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDC) matrix (Sigma) in 70% methanol (HPLC-grade, Sigma) which was applied at 0.06mL/min with a 3mm offset and a velocity of 1200mm/min at 30°C and 10psi using the M5 Sprayer with a heated tray of 50°C. Slides were used immediately or stored in a desiccator until analysis.