Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA253859

Local Sample ID:	c_Ac0_1_TP4i
Subject ID:	SU002617
Subject Type:	Bacteria
Subject Species:	Eggerthella lenta
Taxonomy ID:	84112
Genotype Strain:	DSM 2243, Valencia, AB8n2

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP002623
Sampleprep Summary:	Prior to analysis, intracellular samples were dried at room temperature via Centrivap Benchtop Concentrator (Labconco Corp.). Samples were re-suspended in 60 μL of a chilled solution of 1:1 methanol and acetonitrile, with 24% water at -20oC containing the internal standards CUDA and VAL-TYR-VAL each at 60 ng/mL. Samples were centrifuged at 4°C, 4,122 rcf for 5 minutes and the supernatant transferred to a vial and immediately capped for LC-MS analysis.

Sampleprep ID:

SP002623

Sampleprep Summary:

Prior to analysis, intracellular samples were dried at room temperature via Centrivap Benchtop Concentrator (Labconco Corp.). Samples were re-suspended in 60 μL of a chilled solution of 1:1 methanol and acetonitrile, with 24% water at -20oC containing the internal standards CUDA and VAL-TYR-VAL each at 60 ng/mL. Samples were centrifuged at 4°C, 4,122 rcf for 5 minutes and the supernatant transferred to a vial and immediately capped for LC-MS analysis.