Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA254605

Local Sample ID:	T111
Subject ID:	SU002627
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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MS

Sample Preparation:

Sampleprep ID:	SP002633
Sampleprep Summary:	polar metabolites were extracted from 5µL IF samples using 45µL 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added. Samples were vortexed for 10 min, and centrifuged at maximum speed for 10 min. 30 μL of each extract was removed and dried under nitrogen gas and stored −80°C.

Sampleprep ID:

SP002633

Sampleprep Summary:

polar metabolites were extracted from 5µL IF samples using 45µL 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added. Samples were vortexed for 10 min, and centrifuged at maximum speed for 10 min. 30 μL of each extract was removed and dried under nitrogen gas and stored −80°C.