Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA254725

Local Sample ID:	20221116_QE2_JAS_Job2640_84
Subject ID:	SU002631
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation:

Sampleprep ID:	SP002637
Sampleprep Summary:	We extracted metabolites from 5µL of cell culture media samples using 45µL of a 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix with labelled stable isotope internal standards. Samples in extraction mix were vortexed for 10 min at 4°C and centrifugated at 15,000x rpm for 10 min at 4°C to pellet insoluble material. 20µL of the soluble polar metabolite supernatant was moved to sample vials for analysis by LC-MS.

Sampleprep ID:

SP002637

Sampleprep Summary:

We extracted metabolites from 5µL of cell culture media samples using 45µL of a 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix with labelled stable isotope internal standards. Samples in extraction mix were vortexed for 10 min at 4°C and centrifugated at 15,000x rpm for 10 min at 4°C to pellet insoluble material. 20µL of the soluble polar metabolite supernatant was moved to sample vials for analysis by LC-MS.