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MB Sample ID: SA254734
| Local Sample ID: | T-A11 |
| Subject ID: | SU002632 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Sample Preparation:
| Sampleprep ID: | SP002638 |
| Sampleprep Summary: | 1. Take all samples into a 2 mL EP tube, add 2 steel balls, put them into the tissue grinder, grind at 50 Hz for 60 s, and then homogenize the samples; 2. Accurately weigh 100 mg (±1%) of the homogenized sample into a 2 mL EP tube, add accurately 0.6mL of methanol (including internal standard), and vortex for 30 s 3. Add 2 steel balls, put them into the tissue grinder, and grind for 60 s at 50 Hz; 4. Centrifuge at 4 ℃ for 10 min at 12000 rpm, and the supernatant was filtered through 0.22 µm membrane to obtain the prepared samples for LC-MS; 5. Take 20 µL from each sample to the quality control (QC) samples; (These QC samples were used to monitor deviations of the analytical results from these pool mixtures and compare them to the errors caused by the analytical instrument itself) 6. Use the rest of the samples for LC-MS detection. |
| Sampleprep Protocol Filename: | protocol.pdf |
