Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA256089

Local Sample ID:	D_Serum_20
Subject ID:	SU002650
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP002656
Sampleprep Summary:	50 μL human serum was mixed well, and 50 μL of internal standard solutions (50 nM C17 ceramide for sphingolipid; 200 nM 18:0 D70 PC and 1 μM 16:0 D31-18:1 PE for plasmalogen and PC/PE profiling) were added. Metabolites were extracted from aqueous and organic phases by liquid-liquid extraction after the addition of chloroform and H2O. Nonpolar metabolites containing lipids were collected from the lower organic phase. The organic phases were dried under vacuum and stored at -20℃oC until further analysis. The dried matter was reconstituted with 50 μL of MeOH prior to LC-MS/MS analysis.

Sampleprep ID:

SP002656

Sampleprep Summary:

50 μL human serum was mixed well, and 50 μL of internal standard solutions (50 nM C17 ceramide for sphingolipid; 200 nM 18:0 D70 PC and 1 μM 16:0 D31-18:1 PE for plasmalogen and PC/PE profiling) were added. Metabolites were extracted from aqueous and organic phases by liquid-liquid extraction after the addition of chloroform and H2O. Nonpolar metabolites containing lipids were collected from the lower organic phase. The organic phases were dried under vacuum and stored at -20℃oC until further analysis. The dried matter was reconstituted with 50 μL of MeOH prior to LC-MS/MS analysis.