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MB Sample ID: SA256099
Local Sample ID: | D_Serum_29 |
Subject ID: | SU002650 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP002656 |
Sampleprep Summary: | 50 μL human serum was mixed well, and 50 μL of internal standard solutions (50 nM C17 ceramide for sphingolipid; 200 nM 18:0 D70 PC and 1 μM 16:0 D31-18:1 PE for plasmalogen and PC/PE profiling) were added. Metabolites were extracted from aqueous and organic phases by liquid-liquid extraction after the addition of chloroform and H2O. Nonpolar metabolites containing lipids were collected from the lower organic phase. The organic phases were dried under vacuum and stored at -20℃oC until further analysis. The dried matter was reconstituted with 50 μL of MeOH prior to LC-MS/MS analysis. |