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MB Sample ID: SA256393
Local Sample ID: | Arabic Gum-T24-02-6736 |
Subject ID: | SU002653 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002659 |
Sampleprep Summary: | 300 µL of methanol (Wunstorfer Strasse, Seelze, Germany) was added after the samples were divided into 100 µL Eppendorf tubes, which were then vortexed and incubated at –20 °C for 2 hours. The samples were then vortexed and centrifuged for 15 minutes at 14,000 rpm. The supernatant was then evaporated at 35–40 °C using a speed vacuum evaporator. To assess the analysis's reproducibility, a quality control (QC) sample was created by combining the same volume of each sample. The extract samples were then resuspended in 250 L of Honeywell's LC-MS CHROMASOLV's 0.1% formic acid in Deionized Water (Wunstorfer Strasse, Seelze, Germany). After that, the supernatant was filtered for LC-MS/MS analysis through a hydrophilic nylon syringe filter with a 0.45 m pore size. 100 L of the prepared sample was then gathered in an insert inside LC glass vials. |