Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA256447

Local Sample ID:	Arabic Gum-C34-01-6666
Subject ID:	SU002653
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP002659
Sampleprep Summary:	300 µL of methanol (Wunstorfer Strasse, Seelze, Germany) was added after the samples were divided into 100 µL Eppendorf tubes, which were then vortexed and incubated at –20 °C for 2 hours. The samples were then vortexed and centrifuged for 15 minutes at 14,000 rpm. The supernatant was then evaporated at 35–40 °C using a speed vacuum evaporator. To assess the analysis's reproducibility, a quality control (QC) sample was created by combining the same volume of each sample. The extract samples were then resuspended in 250 L of Honeywell's LC-MS CHROMASOLV's 0.1% formic acid in Deionized Water (Wunstorfer Strasse, Seelze, Germany). After that, the supernatant was filtered for LC-MS/MS analysis through a hydrophilic nylon syringe filter with a 0.45 m pore size. 100 L of the prepared sample was then gathered in an insert inside LC glass vials.

Sampleprep ID:

SP002659

Sampleprep Summary:

300 µL of methanol (Wunstorfer Strasse, Seelze, Germany) was added after the samples were divided into 100 µL Eppendorf tubes, which were then vortexed and incubated at –20 °C for 2 hours. The samples were then vortexed and centrifuged for 15 minutes at 14,000 rpm. The supernatant was then evaporated at 35–40 °C using a speed vacuum evaporator. To assess the analysis's reproducibility, a quality control (QC) sample was created by combining the same volume of each sample. The extract samples were then resuspended in 250 L of Honeywell's LC-MS CHROMASOLV's 0.1% formic acid in Deionized Water (Wunstorfer Strasse, Seelze, Germany). After that, the supernatant was filtered for LC-MS/MS analysis through a hydrophilic nylon syringe filter with a 0.45 m pore size. 100 L of the prepared sample was then gathered in an insert inside LC glass vials.