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MB Sample ID: SA258030
| Local Sample ID: | 2021-11-29_017_D3_E2 |
| Subject ID: | SU002668 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
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Sample Preparation:
| Sampleprep ID: | SP002674 |
| Sampleprep Summary: | Sample prep summary: Islet samples were extracted in 200μL of ice-cold 8:1:1 methanol:water:chloroform, with tissue disruption aided by immediate probe sonication for 20 seconds with a Branson 450 Sonifier set to output level 2, duty cycle 20%. The disrupted samples were allowed to incubate on ice for 10 minutes and were then centrifuged for 5 min at 14,000 xg to pellet cell debris. 180μL of supernatant were dried under a gentle stream of nitrogen gas at room temperature, and were then reconstituted in 45μL of 4:1 water:methanol and transferred to autosampler vials for analysis. Extraction method: Single-phase extraction using 8:1:1 methanol:water:chloroform Extract storage: -80°C until extraction; 4°C after reconstitution |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Single-phase extraction using 8:1:1 methanol:water:chloroform |
| Extract Storage: | -80℃ |
| Sample Resuspension: | -80°C until extraction; 4°C after reconstitution |
