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MB Sample ID: SA267156
| Local Sample ID: | Plasma_PIC_1 |
| Subject ID: | SU002801 |
| Subject Type: | Other organism |
| Subject Species: | Plasmodium berghei |
| Taxonomy ID: | 5823 |
| Genotype Strain: | ANKA |
| Age Or Age Range: | NA |
| Weight Or Weight Range: | NA |
| Height Or Height Range: | NA |
| Gender: | Not applicable |
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Sample Preparation:
| Sampleprep ID: | SP002807 |
| Sampleprep Summary: | Two independent experiments were conducted, each with 6 mice per treatment group. Ice-cold butanol/methanol (1:1) containing 50 µg/mL antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) was added to each plasma sample, as well as a pooled quality control (QC) sample at 10x volume. Samples were vortexed for 10 seconds then snap frozen on dry ice. Thawed samples were sonicated for 15 minutes on ice, stored for 2 hours at -30°C, and then centrifuged for 15 minutes at 16,000×g (4°C). Supernatant was collected, aliquoted, dried down using a vacuum concentrator and stored at -80°C until LC/MS analysis. |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | MeOH/BuOH (1:1) |
| Extract Storage: | -80℃ |
| Sample Resuspension: | NA |
| Sample Derivatization: | NA |
