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MB Sample ID: SA267301
| Local Sample ID: | 03272019_C5_batch15-New_85_NewZMBVNFM-02-E19 |
| Subject ID: | SU002802 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP002808 |
| Sampleprep Summary: | Plasma samples were prepared and analyzed in a randomized order. Plasma was thawed on ice and lipids were extracted using a biphasic separation (ice-cold methanol, methyl tert-butyl ether (MTBE) and water). 260 µl of methanol and 40 ul spike-in standard (cat# 5040156, Sciex, Redwood City, CA, USA) were added to 40 µl of plasma and vortexed for 20 s. Lipids were extracted by adding 1,000 µl of MTBE and incubated under agitation for 30 min at 4°C. Phase separation was induced by adding 250 µl of ice-cold water, followed by vortexing for 1 min and centrifugation at 14,000 g for 15 min at 4°C. The upper phase containing the lipids was collected, dried down under nitrogen, stored at -20°C in 200 ul MeOH. The day of the mass spectrometry acquisition, lipids were dried down under nitrogen and reconstituted with 300 µl of 10 mM ammonium acetate in 9:1 methanol:toluene. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | MTBE |
| Extract Storage: | -20℃ |
