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MB Sample ID: SA270928

Local Sample ID:CRS5
Subject ID:SU002806
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Female

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Sample Preparation:

Sampleprep ID:SP002812
Sampleprep Summary:To precipitate proteins, we combined 200 μl SERUM with 800 μl of methanol, incubated at -20 for 30 min, then centrifuged at 18000 rpm for 10 min. Subsequently, 850 μl of supernatant was transferred to a new tube and evaporated to dryness in a Thermo Savant SpeedVac. Then 200 μl of resuspension solvent methanol was added, compounds were dissolved by vortexing for 5 min and samples were clarified by centrifuged at 18000 rpm for 10 min.
Processing Storage Conditions:-80℃
Extract Storage:-20℃
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